EntroGen’s MGMT promoter methylation and EGFRvIII assays are polymerase chain reaction (PCR)-based and use allele-specific primers in a multiplex reaction to identify their target. The MGMT Methylation Detection Kit works by amplifying the epigenetically silenced MGMT promoter regions after bisulfite treatment of DNA to determine the methylation status of the MGMT promoter. The EGFRvIII Detection Kit provides reagents that detect this mutation in total RNA isolated from tumor biopsies in a single step process (RNA to results in one step). Each kit is supplied with primer/probe sets for multiplexed detection of both target and internal control genes.
Equipment & Materials
EntroGen’s GBM assays require a real-time PCR instrument capable of detecting FAM and VIC fluorescent probes. The tests include reagents required for PCR amplification/detection, as well as validated reaction controls. Columns and reagents for DNA isolation and DNA bisulfite treatment (MGMT assay only) are not included.