K-Ras Mutation Analysis Kit |
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| Real Time PCR kit for detection of exon 2, 3 and 4 mutations of KRAS in human genomic DNA isolated from tumor tissues of patients diagnosed with metastatic colorectal or lung cancers. |
- Certificate: CE-IVD.
- Description: the KRAS gene encodes a small GTPase that plays a key role in transducing signals from EGFR to downstream effectors. KRAS mutations have been commonly found in several types of human malignancies, such as metastatic colorectal cancer (mCRC), lung adenocarcinoma and thyroid cancer. The most common mutations are found in codons 12, 13 and 61. ASCO released its first Provisional Clinical Opinion (PCO) suggesting that all patients to be administered anti-EGFR monoclonal antibody therapy should be screened for KRAS.
- Sample material: DNA isolated tumor biopsies, paraffin-embedded sections (FFPE), or fresh frozen tumors.
- KRAS mutation is detected on FAM channel and IC on the HEX/VIC channel.
- Validated on: ABI (7000, 7300, 7500/Fast, 7900HT, StepOne, StepOne Plus); Rotor-Gene (Q, 3000, 6000); LightCycler 480; Cobas® 4800, Cobas® z480; Bio-Rad CFX96.
