EntroGen’s ctBRAF Mutation Detection Kit is a real-time polymerase chain reaction (PCR)-based assay that uses mutant-specific primers to identify the presence of BRAF V600 mutations. The testing procedure involves three (3) simple steps, which can be completed in approximately 2 hours from DNA isolated from plasma to test results:
- PCR setup with reagents included in the kit
- Amplification and detection using a real-time PCR instrument
- Data analysis
Equipment and Materials
The ctBRAF Mutation Detection Kit requires a real-time PCR instrument capable of detecting FAM and VIC fluorescent probes simultaneously.
This test includes reagents required for the PCR amplification/detection, as well as validated reaction controls. Columns and reagents for cell-free DNA isolation are not included.